High-Coverage Long Read DNA Sequencing with the Oxford Nanopore MinION
Nanopore sequencing was conceived in 1989 by Dave Deamer (UCSC). Over two decades of development from research laboratories and, later on, Oxford Nanopore Technologies resulted in the MinION nanopore sequencer. This work describes the developments in MinION nanopore sequencing and software, and technical milestones achieved since the MinION’s release in 2014. These developments include establishing DNA reads that exceed 200 kb+ lengths and direct, simultaneous detection of nucleotide modifications in DNA and RNA. Due to their portability and real-time aspect, MinIONs are poised to become a routine tool for genomics and biology.