Two-photon fluorescence excitation in continuous-wave infrared optical tweezers.
- Author(s): Liu, Y
- Sonek, GJ
- Berns, MW
- Konig, K
- Tromberg, BJ
- et al.
Published Web Locationhttps://doi.org/10.1364/ol.20.002246
We report the observation of two-photon f luorescence excitation in a continuous-wave (cw) single-beam gradient force optical trap and demonstrate its use as an in situ probe to study the physiological state of an optically confined sample. In particular, a cw Nd:YAG (1064-nm) laser is used simultaneously to confine, and excite visible f luorescence from submicrometer regions of, cell specimens. Two-photon f luorescence emission spectra are presented for motile human sperm cells and immotile Chinese hamster ovary cells that have been labeled with nucleic acid (Propidium Iodide) and pH-sensitive (Snarf) f luorescent probes. The resulting spectra are correlated to light-induced changes in the physiological state experienced by the trapped cells. This spectral technique should prove extremely useful for monitoring cellular activity and the effects of confinement by optical tweezers. © 1995, Optical Society of America.