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Dynamic cell and microparticle control via optoelectronic tweezers

  • Author(s): Ohta, Aaron T.
  • Chiou, Pei-Yu
  • Han, Tae H.
  • Liao, James C.
  • Bhardwaj, Urvashi
  • McCabe, Edward R.B.
  • Yu, Fuqu
  • Sun, Ren
  • Wu, Ming C.
  • et al.

This paper reports on cell and microparticle manipulation using optically induced dielectrophoresis. Our novel optoelectronic tweezers (OET) device enables optically controlled trapping, transportation, and sorting via dielectrophoretic forces. By integrating a spatial light modulator and using direct imaging, arbitrary dynamic manipulation patterns are obtained. Here, we demonstrate manipulation functions, including particle collectors, single-particle traps, individually addressable single-particle arrays, light-defined particle channels, and size-based particle sorting. OET-induced particle manipulation velocities are analyzed as a function of the applied voltage, optical pattern linewidth, and single-particle trap dimensions.

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