A one-pot synthesis of α-l-threofuranosyl nucleoside triphosphates (tNTPs).
- Author(s): Sau, Sujay P
- Chaput, John C
- et al.
Published Web Locationhttp://www.sciencedirect.com/science/article/pii/S0960894X1630542X
TNA (α-l-threofuranosyl nucleoside) triphosphates of adenosine (tATP), guanosine (tGTP), cytidine (tCTP), and thymidine (tTTP) were synthesized from their corresponding 3'-O-phosphoramidite derivatives using a novel one-pot reaction that is less moisture sensitive than traditional methods. The chemically synthesized tNTPs, despite containing an unnatural 3'-triphosphate moiety, are similar in thermal stability to natural nucleotide triphosphates.