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Weak base pairing in both seed and 3' regions reduces RNAi off-targets and enhances si/shRNA designs

Published Web Location

http://europepmc.org/articles/PMC4231738?pdf=render
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Abstract

© The Author(s) 2014.The use of RNA interference is becoming routine in scientific discovery and treatment of human disease. However, its applications are hampered by unwanted effects, particularly off-targeting through miRNA-like pathways. Recent studies

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