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Protocol for assessing endolysosomal chloride channel function in mammalian cells using endolysosomal patch-clamp technique
Published Web Location
https://pmc.ncbi.nlm.nih.gov/articles/PMC13579410/No data is associated with this publication.
Abstract
Here, we present a protocol for assessing endolysosomal chloride channel function in mammalian cells using the endolysosomal patch-clamp technique. We describe steps for vesicle enlargement, fabrication and fire-polishing of high-resistance pipettes, and mechanical dissection and isolation of enlarged vesicles. We then detail procedures for giga-seal formation and configuration to whole-endolysosome modes. For complete details on the use and execution of this protocol, please refer to Wang et al.1.
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