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Open Access Publications from the University of California

Swabs to genomes: A comprehensive workflow

  • Author(s): Dunitz, MI
  • Lang, JM
  • Jospin, G
  • Darling, AE
  • Eisen, JA
  • Coil, DA
  • et al.

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© 2015 Dunitz et al. The sequencing, assembly, and basic analysis of microbial genomes, once a painstaking and expensive undertaking, has become much easier for research labs with access to standard molecular biology and computational tools. However, there are a confusing variety of options available for DNA library preparation and sequencing, and inexperience with bioinformatics can pose a significant barrier to entry for many who may be interested in microbial genomics. The objective of the present study was to design, test, troubleshoot, and publish a simple, comprehensive workflow from the collection of an environmental sample (a swab) to a published microbial genome; empowering even a lab or classroom with limited resources and bioinformatics experience to performit.

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