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Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA.
- Author(s): Liu, Jie
- Doty, Tammy
- Gibson, Bryan
- Heyer, Wolf-Dietrich
- et al.
Published Web Location
https://doi.org/10.1038/nsmb.1904Abstract
BRCA2 is a tumor suppressor that functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2 and show that it binds to ~6 RAD51 molecules and promotes RAD51 binding to ssDNA coated by replication protein A (RPA), in a manner that is stimulated by DSS1.
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