UC Irvine

Nanoparticle mediated targeted delivery has been widely studied as it provides nanoparticle-cell surface interaction and helps shift biodistribution to diseased cells, thereby overcoming the disadvantages of conventional drug delivery methods. Fluorescent proteins have been used as markers to detect protein-protein interaction and can be used to track the movements of other proteins. The first part of this study aimed to produce fluorescent proteins at high yield, so we studied mOx GFP and codon optimized it for yeast. The second part of this study dealt with nanospringy linkers, specifically flagelliform, and its construction. Springy linkers are designed to reduce mechanical forces on bonds produced by the nanoparticle, which will stabilize adhesion. In previous studies, springy linkers with fewer amino acids base lengths were already constructed, and we investigated if we could construct them with high amino acid count. Springy linkers will pave the way to experiments to validate our theory.