Tracking the secondary photodynamics of the green/red cyanobacteriochrome RcaE from Fremyella diplosiphon
- Author(s): Chang, CW
- Gottlieb, SM
- Rockwell, NC
- Martin, SS
- Lagarias, JC
- Larsen, DS
- et al.
Published Web Locationhttps://doi.org/10.1016/j.cplett.2015.11.059
© 2015 Elsevier B.V. All rights reserved. Cyanobacteriochrome RcaE regulates Type III complementary chromatic adaption in the cyanobacterium Fremyella diplosiphon by photoswitching between a green-absorbing dark state (15ZPg) and red-absorbing photoproduct (15EPr). Ultrafast photodynamics of RcaE involve tautomerization of the bilin chromophore, inhomogeneity, and the generation of three primary photointermediates in the forward reaction (Lumi-Go, Lumi-Gr, and Lumi-Gf). The secondary photodynamics reported here show that only Lumi-Goevolves to15EPrvia spectrally similar Meta-Go1and Meta-Go2intermediates, with a protonation reaction occurring at the final step on the millisecond timescale. Reverse reaction dynamics were characterized and reveal an unusually long-lived Lumi-Rfphotoproduct and a blue-shifted Meta-Ryintermediate.
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