Lawrence Berkeley National Laboratory
Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples.
- Author(s): Yu, Feiqiao Brian
- Blainey, Paul C
- Schulz, Frederik
- Woyke, Tanja
- Horowitz, Mark A
- Quake, Stephen R
- et al.
Published Web Locationhttps://doi.org/10.7554/elife.26580
Metagenomics and single-cell genomics have enabled genome discovery from unknown branches of life. However, extracting novel genomes from complex mixtures of metagenomic data can still be challenging and represents an ill-posed problem which is generally approached with ad hoc methods. Here we present a microfluidic-based mini-metagenomic method which offers a statistically rigorous approach to extract novel microbial genomes while preserving single-cell resolution. We used this approach to analyze two hot spring samples from Yellowstone National Park and extracted 29 new genomes, including three deeply branching lineages. The single-cell resolution enabled accurate quantification of genome function and abundance, down to 1% in relative abundance. Our analyses of genome level SNP distributions also revealed low to moderate environmental selection. The scale, resolution, and statistical power of microfluidic-based mini-metagenomics make it a powerful tool to dissect the genomic structure of microbial communities while effectively preserving the fundamental unit of biology, the single cell.