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Open Access Publications from the University of California

Production of tetra-methylpyrazine using engineered Corynebacterium glutamicum.

  • Author(s): Eng, Thomas
  • Sasaki, Yusuke
  • Herbert, Robin A
  • Lau, Andrew
  • Trinh, Jessica
  • Chen, Yan
  • Mirsiaghi, Mona
  • Petzold, Christopher J
  • Mukhopadhyay, Aindrila
  • et al.

Corynebacterium glutamicum ATCC 13032 is an established and industrially-relevant microbial host that has been utilized for the expression of many desirable bioproducts. Tetra-methylpyrazine (TMP) is a naturally occurring alkylpyrazine with broad applications spanning fragrances to resins. We identified an engineered strain of C. glutamicum which produces 5 ​g/L TMP and separately, a strain which can co-produce both TMP and the biofuel compound isopentenol. Ionic liquids also stimulate TMP production in engineered strains. Using a fed batch-mode feeding strategy, ionic liquid stimulated strains produced 2.2 ​g/L of tetra-methylpyrazine. We show that feedback from a specific heterologous gene pathway on host physiology leads to acetoin accumulation and the production of TMP.

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