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Open Access Publications from the University of California

Multiscale modeling of a red blood cell and its fluid- structure interaction

  • Author(s): Peng, Zhangli
  • et al.
Abstract

We develop a three-level multiscale approach of the red blood cell (RBC) membrane and couple this approach with a boundary element method (BEM) for the surrounding Stokes flow to simulate the mechanical behavior of a RBC under various in vitro and in vivo conditions. Our multiscale approach of this membrane includes three models: in the whole cell level (Level III), a finite element method (FEM) is employed to model the lipid bilayer and the cytoskeleton as two distinct layers of shells with sliding -only interaction. The mechanical properties of the cytoskeleton are obtained from a coarse-grained molecular dynamics model (Level II) of the junctional complex. The spectrin, a major protein of the cytoskeleton, is simulated using a molecular-based constitutive model (Level I), including its domain folding/unfolding reactions. A BEM of the surrounding Stokes flow is coupled with the FEM model of the membrane through a staggered coupling algorithm. Using this method, we first predict the resting shapes of healthy and diseased RBCs. Secondly, we simulate three quasi-static experiments of the micropipette aspiration, the optical tweezer stretching, and the flow channel stretching. Detailed distributions of the bilayer-skeleton interaction force that may cause their dissociation and lead to phenomena such as vesiculation are predicted. Specifically, our model predicts a correlation between the occurrence of spectrin unfolding and increase in the mechanical load upon individual bilayer-skeleton pinning points in micropipette aspirations. A simulation of the necking process after bilayer-skeleton dissociation is also conducted. Thirdly, we study RBC dynamics in capillary flow and find that the skeleton density is large near the vessel wall, and the maximum bilayer-skeleton interaction force occurs at the trailing edge. Finally, we investigate the tumbling, tank- treading, and swinging motions of RBCs in shear flow. The dependencies of tank-treading frequency on the blood plasma viscosity and the membrane viscosity we found match well with the existing experimental and computational data. The simulation results show that during tank- treading there is almost no protein density variation of the skeleton due to the significant bilayer-skeleton friction. The distributions of shear deformation, bilayer- skeleton interaction forces are also predicted

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