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Fiber-optic chemical sensors for competitive binding fluoroimmunoassay.

  • Author(s): Tromberg, BJ;
  • Sepaniak, MJ;
  • Vo-Dinh, T;
  • Griffin, GD
  • et al.
Abstract

This paper describes the development of a fiber-optic chemical sensor based on the principle of competitive-binding fluorescence immunoassay. Rabbit immunoglobln G (IgG) is covalently Immobilized on the distal sensing tip of a quartz optical fiber. The sensor is exposed to fluorescein Isothiocyanate (FITC) labeled and unlabeled anti-rabbit IgG. The 488-nm line of an argon-ion laser provides excitation of sensor-bound analyte. This results in fluorescence emission at the optical fiber‧s sensing tip. Sensor response is inversely proportional to the amount of unlabeled anti-IgG In the sample. Limits of detection (LOD) vary with Incubation time, sample size, and measurement conditions. For 10-μL samples, typical LOD are 25 fmol of unlabeled antibody In a 20-min Incubation period. These results Indicate that each fiber-optic fluoroimmunosensor can be constructed to perform a single sensitive, rapid, low-volume immunoassay, in in situ or benchtop applications. © 1987, American Chemical Society. All rights reserved.

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