Skip to main content
eScholarship
Open Access Publications from the University of California

A minimalist biosensor: Quantitation of cyclic di-GMP using the conformational change of a riboswitch aptamer

  • Author(s): Kellenberger, CA
  • Sales-Lee, J
  • Pan, Y
  • Gassaway, MM
  • Herr, AE
  • Hammond, MC
  • et al.
Abstract

© 2015 Taylor and Francis Group, LLC. Cyclic di-GMP (c-di-GMP) is a second messenger that is important in regulating bacterial physiology and behavior, including motility and virulence. Many questions remain about the role and regulation of this signaling molecule, but current methods of detection are limited by either modest sensitivity or requirements for extensive sample purification. We have taken advantage of a natural, high affinity receptor of c-di-GMP, the Vc2 riboswitch aptamer, to develop a sensitive and rapid electrophoretic mobility shift assay (EMSA) for c-di-GMP quantitation that required minimal engineering of the RNA.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View