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Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media
- Rajput, Akanksha;
- Poudel, Saugat;
- Tsunemoto, Hannah;
- Meehan, Michael;
- Szubin, Richard;
- Olson, Connor A;
- Seif, Yara;
- Lamsa, Anne;
- Dillon, Nicholas;
- Vrbanac, Alison;
- Sugie, Joseph;
- Dahesh, Samira;
- Monk, Jonathan M;
- Dorrestein, Pieter C;
- Knight, Rob;
- Pogliano, Joe;
- Nizet, Victor;
- Feist, Adam M;
- Palsson, Bernhard O
Abstract
The evolving antibiotic-resistant behavior of health care-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more. The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments. The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.
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