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Open Access Publications from the University of California

Identifying the effect of vancomycin on health care-associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media.

  • Author(s): Rajput, Akanksha
  • Poudel, Saugat
  • Tsunemoto, Hannah
  • Meehan, Michael
  • Szubin, Richard
  • Olson, Connor A
  • Seif, Yara
  • Lamsa, Anne
  • Dillon, Nicholas
  • Vrbanac, Alison
  • Sugie, Joseph
  • Dahesh, Samira
  • Monk, Jonathan M
  • Dorrestein, Pieter C
  • Knight, Rob
  • Pogliano, Joe
  • Nizet, Victor
  • Feist, Adam M
  • Palsson, Bernhard O
  • et al.


The evolving antibiotic-resistant behavior of health care-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more.


The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments.


The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.

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