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Identifying the effect of vancomycin on health care-associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media.
- Author(s): Rajput, Akanksha
- Poudel, Saugat
- Tsunemoto, Hannah
- Meehan, Michael
- Szubin, Richard
- Olson, Connor A
- Seif, Yara
- Lamsa, Anne
- Dillon, Nicholas
- Vrbanac, Alison
- Sugie, Joseph
- Dahesh, Samira
- Monk, Jonathan M
- Dorrestein, Pieter C
- Knight, Rob
- Pogliano, Joe
- Nizet, Victor
- Feist, Adam M
- Palsson, Bernhard O
- et al.
Published Web Location
https://doi.org/10.1093/gigascience/giaa156Abstract
Background
The evolving antibiotic-resistant behavior of health care-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more.Findings
The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments.Conclusions
The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.