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Telomere Region Ensures Full Encapsulation of Mitotic Chromosomes


During mitosis, nuclear envelope (NE) disassembles to proceed nuclear division and reassembles to encapsulate the newly generated diploid chromosome set into the daughter nucleus. A failure of this compartmentalization gives rise to micronuclei, leading to DNA damage and chromothripsis which can be a cause of disease. While the secure compartmentalization of the complete chromosome set into the single cell organelle unit is a fundamental biological paradigm, the exact mechanism behind this principle remains largely unknown. Using high-temporal microscopy, we discovered that nuclear filament protein Lamin B1 localizes and spreads from the telomere region of condensed chromosomes. The absence of immediate chromosome de-condensation regulated by barrier-to-autointegration factor (BAF) results in mis-shaped nuclei in daughter cells, while delocalization of Lamin B1 from telomere region results in massive micro-nuclei formation. Our findings reveal a new paradigm of full mitotic chromosome encapsulation that relies on both attraction of nuclear filament protein to telomere region and exclusion due to chromosome de-condensation during telophase.

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