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Short-chain ketone production by engineered polyketide synthases in Streptomyces albus

  • Author(s): Yuzawa, S
  • Mirsiaghi, M
  • Jocic, R
  • Fujii, T
  • Masson, F
  • Benites, VT
  • Baidoo, EEK
  • Sundstrom, E
  • Tanjore, D
  • Pray, TR
  • George, A
  • Davis, RW
  • Gladden, JM
  • Simmons, BA
  • Katz, L
  • Keasling, JD
  • et al.
Abstract

Microbial production of fuels and commodity chemicals has been performed primarily using natural or slightly modified enzymes, which inherently limits the types of molecules that can be produced. Type I modular polyketide synthases (PKSs) are multi-domain enzymes that can produce unique and diverse molecular structures by combining particular types of catalytic domains in a specific order. This catalytic mechanism offers a wealth of engineering opportunities. Here we report engineered microbes that produce various short-chain (C5-C7) ketones using hybrid PKSs. Introduction of the genes into the chromosome of Streptomyces albus enables it to produce >1 g · l-1 of C6 and C7 ethyl ketones and several hundred mg · l-1 of C5 and C6 methyl ketones from plant biomass hydrolysates. Engine tests indicate these short-chain ketones can be added to gasoline as oxygenates to increase the octane of gasoline. Together, it demonstrates the efficient and renewable microbial production of biogasolines by hybrid enzymes.

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