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Automated assessment of pavlovian conditioned freezing and shock reactivity in mice using the video freeze system.

  • Author(s): Anagnostaras, Stephan G
  • Wood, Suzanne C
  • Shuman, Tristan
  • Cai, Denise J
  • Leduc, Arthur D
  • Zurn, Karl R
  • Zurn, J Brooks
  • Sage, Jennifer R
  • Herrera, Gerald M
  • et al.
Abstract

The Pavlovian conditioned freezing paradigm has become a prominent mouse and rat model of learning and memory, as well as of pathological fear. Due to its efficiency, reproducibility and well-defined neurobiology, the paradigm has become widely adopted in large-scale genetic and pharmacological screens. However, one major shortcoming of the use of freezing behavior has been that it has required the use of tedious hand scoring, or a variety of proprietary automated methods that are often poorly validated or difficult to obtain and implement. Here we report an extensive validation of the Video Freeze system in mice, a "turn-key" all-inclusive system for fear conditioning in small animals. Using digital video and near-infrared lighting, the system achieved outstanding performance in scoring both freezing and movement. Given the large-scale adoption of the conditioned freezing paradigm, we encourage similar validation of other automated systems for scoring freezing, or other behaviors.

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