- Main
A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice
- Wierbowski, Shayne D;
- Vo, Tommy V;
- Falter-Braun, Pascal;
- Jobe, Timothy O;
- Kruse, Lars H;
- Wei, Xiaomu;
- Liang, Jin;
- Meyer, Michael J;
- Akturk, Nurten;
- Rivera-Erick, Christen A;
- Cordero, Nicolas A;
- Paramo, Mauricio I;
- Shayhidin, Elnur E;
- Bertolotti, Marta;
- Tippens, Nathaniel D;
- Akther, Kazi;
- Sharma, Rita;
- Katayose, Yuichi;
- Salehi-Ashtiani, Kourosh;
- Hao, Tong;
- Ronald, Pamela C;
- Ecker, Joseph R;
- Schweitzer, Peter A;
- Kikuchi, Shoshi;
- Mizuno, Hiroshi;
- Hill, David E;
- Vidal, Marc;
- Moghe, Gaurav D;
- McCouch, Susan R;
- Yu, Haiyuan
- et al.
Published Web Location
https://doi.org/10.1073/pnas.1918068117Abstract
Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.
Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.
Main Content
Enter the password to open this PDF file:
-
-
-
-
-
-
-
-
-
-
-
-
-
-