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Open Access Publications from the University of California

Poly(N-isopropylacrylamide)-gated Fe3O4/SiO2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme

  • Author(s): Yu, E
  • Galiana, I
  • Martínez-Máñez, R
  • Stroeve, P
  • Marcos, MD
  • Aznar, E
  • Sancenón, F
  • Murguía, JR
  • Amorós, P
  • et al.

© 2015 Elsevier B.V. Core-shell nanoparticles comprised of Fe3O4 cores and a mesoporous silica shell with an average expanded pore size of 6.07nm and coated with a poly(N-isopropylacrylamide) (PNIPAM) layer (CS-MSNs-EP-PNIPAM) were prepared and characterized. The nanoparticles was loaded with (Ru(bipy)32+) dye or an antibacterial enzyme, lysozyme, to obtain CS-MSNs-EP-PNIPAM-Ru(bipy)32+ and CS-MSNs-EP-PNIPAM-Lys, respectively. The lysozyme loading was determined to be 160mg/g of nanoparticle. It was seen that Ru(bipy)32+ and lysozyme release was minimal at a room temperature of 25°C while at physiological temperature (37°C), abrupt release was observed. The applicability of the CS-MSNs-EP-PNIPAM-Lys was further tested with two Gram-positive bacteria samples, Bacillus cereus and Micrococcus luteus. At physiological temperature, the nanoparticles were shown to reduce bacterial growth, indicating a successful release of lysozyme from the nanoparticles. This nanoparticle system shows potential as a nanocarrier for the loading of similarly sized proteins or other species as a drug delivery platform.

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