Skip to main content
eScholarship
Open Access Publications from the University of California

New shuttle vectors for ectopic insertion of genes into Bacillus subtilis

  • Author(s): Middleton, R
  • Hofmeister, A
  • et al.
Abstract

We have constructed shuttle vectors for integration of genes via double homologous recombination into three ectopic sites on the chromosome of Bacillus subtilis. The sites of integration are the pyrD, gltA, and sacA genes located at 139degrees, 172degrees, and 333degrees, respectively, on the chromosome. Integration of the vectors into the target genes leads to antibiotic resistance as well as different metabolic phenotypes. B. subtilis strains with integrations of the empty vectors were able to sporulate at rates comparable to wild type cells. Similar levels of expression were obtained from constitutive lacZ fusions integrated at the different sites. (C) 2004 Elsevier Inc. All rights reserved.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View