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De novo DNA synthesis using polymerase-nucleotide conjugates
- Palluk, Sebastian;
- Arlow, Daniel H;
- de Rond, Tristan;
- Barthel, Sebastian;
- Kang, Justine S;
- Bector, Rathin;
- Baghdassarian, Hratch M;
- Truong, Alisa N;
- Kim, Peter W;
- Singh, Anup K;
- Hillson, Nathan J;
- Keasling, Jay D
Published Web Location
https://doi.org/10.1038/nbt.4173Abstract
An enzymatic approach enables synthesis of a defined DNA sequence using TdT with reversibly tethered dNTPs.
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