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Phased diploid genome assembly with single-molecule real-time sequencing
- Chin, Chen-Shan;
- Peluso, Paul;
- Sedlazeck, Fritz J;
- Nattestad, Maria;
- Concepcion, Gregory T;
- Clum, Alicia;
- Dunn, Christopher;
- O'Malley, Ronan;
- Figueroa-Balderas, Rosa;
- Morales-Cruz, Abraham;
- Cramer, Grant R;
- Delledonne, Massimo;
- Luo, Chongyuan;
- Ecker, Joseph R;
- Cantu, Dario;
- Rank, David R;
- Schatz, Michael C
Published Web Location
https://doi.org/10.1038/nmeth.4035Abstract
The open-source FALCON and FALCON-Unzip software utilize long-read sequencing data to generate contiguous, accurate and phased diploid assemblies, even from genomes that are highly heterozygous.
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