The Need for Integrated Approaches in Metabolic Engineering.
- Author(s): Lechner, Anna
- Brunk, Elizabeth
- Keasling, Jay D
- et al.
Published Web Locationhttp://cshperspectives.cshlp.org/content/8/11/a023903.long
This review highlights state-of-the-art procedures for heterologous small-molecule biosynthesis, the associated bottlenecks, and new strategies that have the potential to accelerate future accomplishments in metabolic engineering. We emphasize that a combination of different approaches over multiple time and size scales must be considered for successful pathway engineering in a heterologous host. We have classified these optimization procedures based on the "system" that is being manipulated: transcriptome, translatome, proteome, or reactome. By bridging multiple disciplines, including molecular biology, biochemistry, biophysics, and computational sciences, we can create an integral framework for the discovery and implementation of novel biosynthetic production routes.