The clinical management of bone defects caused by trauma or nonunion fractures remains a challenge in orthopedic practice due to the poor integration and biocompatibility properties of the scaffold or implant material. In the current work, the osteogenic properties of carboxyl-modified single-walled carbon nanotubes (COOH-SWCNTs) were investigated in vivo and in vitro. When human preosteoblasts and murine embryonic stem cells were cultured on coverslips sprayed with COOH-SWCNTs, accelerated osteogenic differentiation was manifested by increased expression of classical bone marker genes and an increase in the secretion of osteocalcin, in addition to prior mineralization of the extracellular matrix. These results predicated COOH-SWCNTs' use to further promote osteogenic differentiation in vivo. In contrast, both cell lines had difficulties adhering to multi-walled carbon nanotube-based scaffolds, as shown by scanning electron microscopy. While a suspension of SWCNTs caused cytotoxicity in both cell lines at levels >20 μg/mL, these levels were never achieved by release from sprayed SWCNTs, warranting the approach taken. In vivo, human allografts formed by the combination of demineralized bone matrix or cartilage particles with SWCNTs were implanted into nude rats, and ectopic bone formation was analyzed. Histological analysis of both types of implants showed high permeability and pore connectivity of the carbon nanotube-soaked implants. Numerous vascularization channels appeared in the formed tissue, additional progenitor cells were recruited, and areas of de novo ossification were found 4 weeks post-implantation. Induction of the expression of bone-related genes and the presence of secreted osteopontin protein were also confirmed by quantitative polymerase chain reaction analysis and immunofluorescence, respectively. In summary, these results are in line with prior contributions that highlight the suitability of SWCNTs as scaffolds with high bone-inducing capabilities both in vitro and in vivo, confirming them as alternatives to current bone-repair therapies.

Associated production of bottomonia and open charm hadrons in pp collisions at s=7 and 8 TeV is observed using data corresponding to an integrated luminosity of 3 fb−1 accumulated with the LHCb detector. The observation of five combinations, Y(1S)D0, Y(2S)D0, Y(1S)D+, Y(2S)D+ and Y(1S)Ds+, is reported. Production crosssections are measured for Y(1S)D0 and Y(1S)D+ pairs in the forward region. The measured cross-sections and the differential distributions indicate the dominance of double parton scattering as the main production mechanism.[Figure not available: see fulltext.]

The difference between the CP asymmetries in the decays Λc+ → pK−K+ and Λc+ → pπ−π+ is presented. Proton-proton collision data taken at centre-of-mass energies of 7 and 8 TeV collected by the LHCb detector in 2011 and 2012 are used, corresponding to an integrated luminosity of 3 fb−1. The Λc+ candidates are reconstructed as part of the Λb0 → Λc+μ−X decay chain. In order to maximize the cancellation of production and detection asymmetries in the difference, the final-state kinematic distributions of the two samples are aligned by applying phase-space-dependent weights to the Λc+ → pπ−π+ sample. This alters the definition of the integrated CP asymmetry to ACPwgt(pπ−π+). Both samples are corrected for reconstruction and selection efficiencies across the five-dimensional Λc+ decay phase space. The difference in CP asymmetries is found to be ΔACPwgt=ACP(pK−K+)−ACPwgt(pπ−π+)=(0.30±0.91±0.61)%, where the first uncertainty is statistical and the second is systematic.[Figure not available: see fulltext.].

A search for the non-resonant decays Bs0 → μ+μ−μ+μ− and B0 → μ+μ−μ+μ− is presented. The measurement is performed using the full Run 1 data set collected in proton-proton collisions by the LHCb experiment at the LHC. The data correspond to integrated luminosities of 1 and 2 fb−1 collected at centre-of-mass energies of 7 and 8 TeV, respectively. No signal is observed and upper limits on the branching fractions of the non-resonant decays at 95% confidence level are determined to beℬ(Bs0→μ+μ−μ+μ−)<2.5×10−9,[Figure not available: see fulltext.]

The first observation of the B_{s}^{0}→D[over ¯]^{0}K_{S}^{0} decay mode and evidence for the B_{s}^{0}→D[over ¯]^{*0}K_{S}^{0} decay mode are reported. The data sample corresponds to an integrated luminosity of 3.0  fb^{-1} collected in pp collisions by LHCb at center-of-mass energies of 7 and 8 TeV. The branching fractions are measured to be B(B_{s}^{0}→D[over ¯]^{0}K[over ¯]^{0})=[4.3±0.5(stat)±0.3(syst)±0.3(frag)±0.6(norm)]×10^{-4},B(B_{s}^{0}→D[over ¯]^{*0}K[over ¯]^{0})=[2.8±1.0(stat)±0.3(syst)±0.2(frag)±0.4(norm)]×10^{-4},where the uncertainties are due to contributions coming from statistical precision, systematic effects, and the precision of two external inputs, the ratio f_{s}/f_{d} and the branching fraction of B^{0}→D[over ¯]^{0}K_{S}^{0}, which is used as a calibration channel.