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High-throughput, quantitative analyses of genetic interactions in E. coli
- Typas, Athanasios;
- Nichols, Robert J;
- Siegele, Deborah A;
- Shales, Michael;
- Collins, Sean R;
- Lim, Bentley;
- Braberg, Hannes;
- Yamamoto, Natsuko;
- Takeuchi, Rikiya;
- Wanner, Barry L;
- Mori, Hirotada;
- Weissman, Jonathan S;
- Krogan, Nevan J;
- Gross, Carol A
Published Web Location
https://doi.org/10.1038/nmeth.1240Abstract
An array-based high-throughput approach, genetic interaction analysis technology for Escherichia coli (GIANT-coli), now allows comprehensive genetic interaction screens in bacteria. The method uses bacterial conjugation and robotic technology to generate double mutants on a genome-wide scale. In this issue another paper presents eSGA, a very similar approach.
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