Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA

2023

Published Web Location

https://doi.org/10.1039/d3cc00043e

Abstract

We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.

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Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA

Published Web Location