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Peroxiporin expression is an important factor for cancer cell susceptibility to therapeutic H2O2: Implications for pharmacological ascorbate therapy

  • Author(s): Erudaitius, D
  • Huang, A
  • Kazmi, S
  • Buettner, GR
  • Rodgers, VGJ
  • et al.
Abstract

© 2017 Erudaitius et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Cancer cell toxicity to therapeutic H2O2varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H2O2doses (P< 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.

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