Dual genetically encoded phage-displayed ligands
- Author(s): Mohan, K
- Weiss, GA
- et al.
Published Web Locationhttps://doi.org/10.1016/j.ab.2014.02.025
M13 bacteriophage display presents polypeptides as fusions to phage coat proteins. Such phage-displayed ligands offer useful reagents for biosensors. Here, we report a modified phage propagation protocol for the consistent and robust display of two different genetically encoded ligands on the major coat protein, P8. The results demonstrate that the phage surface reaches a saturation point for maximum peptide display. © 2014 Elsevier Inc. All rights reserved.
Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.