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Dual genetically encoded phage-displayed ligands

  • Author(s): Mohan, K
  • Weiss, GA
  • et al.
Abstract

M13 bacteriophage display presents polypeptides as fusions to phage coat proteins. Such phage-displayed ligands offer useful reagents for biosensors. Here, we report a modified phage propagation protocol for the consistent and robust display of two different genetically encoded ligands on the major coat protein, P8. The results demonstrate that the phage surface reaches a saturation point for maximum peptide display. © 2014 Elsevier Inc. All rights reserved.

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