Skip to main content
Open Access Publications from the University of California

UC Irvine

UC Irvine Previously Published Works bannerUC Irvine

Design and Synthesis of an Alkynyl Luciferin Analogue for Bioluminescence Imaging.

  • Author(s): Steinhardt, Rachel C;
  • O'Neill, Jessica M;
  • Rathbun, Colin M;
  • McCutcheon, David C;
  • Paley, Miranda A;
  • Prescher, Jennifer A
  • et al.

Herein, the synthesis and characterization of an alkyne-modified luciferin is reported. This bioluminescent probe was accessed using C-H activation methodology and was found to be stable in solution and capable of light production with firefly luciferase. The luciferin analogue was also cell permeant and emitted more redshifted light than d-luciferin, the native luciferase substrate. Based on these features, the alkynyl luciferin will be useful for a variety of imaging applications.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View