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Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas.
- Author(s): Riegler, Johannes
- Ebert, Antje
- Qin, Xulei
- Shen, Qi
- Wang, Mouer
- Ameen, Mohamed
- Kodo, Kazuki
- Ong, Sang-Ging
- Lee, Won Hee
- Lee, Grace
- Neofytou, Evgenios
- Gold, Joseph D
- Connolly, Andrew J
- Wu, Joseph C
- et al.
Published Web Location
https://doi.org/10.1016/j.stemcr.2015.12.008Abstract
The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm(3). A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm(3) and with a sensitivity of more than 87%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking.
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