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A fast and efficient procedure to produce scFvs specific for large macromolecular complexes
Published Web Location
https://doi.org/10.1016/j.jim.2006.10.005Abstract
We have expanded the application of antibody phage display to a new type of antigen: ribonucleoprotein (RNP) complexes. We describe a simple and efficient method for screening antibodies specific for large intact RNPs and individual components. We also describe a fast and easy method to overcome the abundance of amber stop codons in the positive phage clones. The resulting antibodies have been used in ELISA and Western blot analysis.
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