Skip to main content
eScholarship
Open Access Publications from the University of California

Microfluidics and Metabolomics Reveal Symbiotic Bacterial-Fungal Interactions Between Mortierella elongata and Burkholderia Include Metabolite Exchange.

  • Author(s): Uehling, Jessie K
  • Entler, Matthew R
  • Meredith, Hannah R
  • Millet, Larry J
  • Timm, Collin M
  • Aufrecht, Jayde A
  • Bonito, Gregory M
  • Engle, Nancy L
  • Labbé, Jessy L
  • Doktycz, Mitchel J
  • Retterer, Scott T
  • Spatafora, Joseph W
  • Stajich, Jason E
  • Tschaplinski, Timothy J
  • Vilgalys, Rytas J
  • et al.
Abstract

We identified two poplar (Populus sp.)-associated microbes, the fungus, Mortierella elongata strain AG77, and the bacterium, Burkholderia strain BT03, that mutually promote each other's growth. Using culture assays in concert with a novel microfluidic device to generate time-lapse videos, we found growth specific media differing in pH and pre-conditioned by microbial growth led to increased fungal and bacterial growth rates. Coupling microfluidics and comparative metabolomics data results indicated that observed microbial growth stimulation involves metabolic exchange during two ordered events. The first is an emission of fungal metabolites, including organic acids used or modified by bacteria. A second signal of unknown nature is produced by bacteria which increases fungal growth rates. We find this symbiosis is initiated in part by metabolic exchange involving fungal organic acids.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
Current View