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Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

et al.

Published Web Location

https://doi.org/10.1038/s41592-019-0401-3
Abstract

We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations.

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