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Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution.

  • Author(s): Yang, Bin
  • Chen, Xingye
  • Wang, Yina
  • Feng, Siyu
  • Pessino, Veronica
  • Stuurman, Nico
  • Cho, Nathan
  • Cheng, Karen
  • Lord, Samuel
  • Xu, Linfeng
  • Xu, Linfeng
  • Xie, Dan
  • Mullins, R
  • Leonetti, Manuel
  • Huang, Bo
  • et al.
Abstract

We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations.

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