Skip to main content
Microprocessor-controlled photon-counting spectrofluorometer
Abstract
The construction details and performance characteristics for a fluorescence spectrophotometer are described. The system utilizes proton counting for signal detection and an Apple II microcomputer for data acquisition and analysis. Data-acquisition techniques are given along with typical excitation, emission, and polarization spectra. Particular reference is made to application of the instrument to fluorescence studies of protein/polymer films.
Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.