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Microprocessor-controlled photon-counting spectrofluorometer

Creative Commons 'BY' version 4.0 license
Abstract

The construction details and performance characteristics for a fluorescence spectrophotometer are described. The system utilizes proton counting for signal detection and an Apple II microcomputer for data acquisition and analysis. Data-acquisition techniques are given along with typical excitation, emission, and polarization spectra. Particular reference is made to application of the instrument to fluorescence studies of protein/polymer films.

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