Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.
- Author(s): Battisti, Antonella
- Digman, Michelle A
- Gratton, Enrico
- Storti, Barbara
- Beltram, Fabio
- Bizzarri, Ranieri
- et al.
Published Web Locationhttps://doi.org/10.1039/c2cc30373f
A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.