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Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.

  • Author(s): Battisti, Antonella
  • Digman, Michelle A
  • Gratton, Enrico
  • Storti, Barbara
  • Beltram, Fabio
  • Bizzarri, Ranieri
  • et al.
Abstract

A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.

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