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Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging
Abstract
A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.
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