Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

2012

Published Web Location

https://doi.org/10.1039/c2cc30373f

Creative Commons 'BY' version 4.0 license

Abstract

A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.

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Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

Published Web Location