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Advanced methods of microscope control using μManager software.

  • Author(s): Edelstein, Arthur D
  • Tsuchida, Mark A
  • Amodaj, Nenad
  • Pinkard, Henry
  • Vale, Ronald D
  • Stuurman, Nico
  • et al.
Abstract

μManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, μManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.

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