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Rolling circle amplification of complete nematode mitochondrial genomes

  • Author(s): Hyman, Bradley C.
  • et al.
Abstract

To enable investigation of nematode mitochondrial DNA evolution, methodology has been developed to amplify intact nematode mitochondrial genomes in preparative yields using a rolling circle amplification strategy. Successful reactions were generated from whole cell template DNA prepared by alkaline lysis. Rolling circle amplification products can be used as templates for standard PCR reactions, restriction enzyme reactions, and direct DNA sequencing.

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