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Effect of in vivo ozone exposure on in vitro pulmonary alveolar macrophage mobility

  • Author(s): McAllen, SJ
  • Chiu, SP
  • Phalen, RF
  • Rasmussen, RE
  • et al.
Abstract

The effect of in vivo 03exposure on the mobility of pulmonary alveolar macrophages (PAM) in vitro was investigated. Eight randomly selected rats were exposed for 4 h. Four rats were exposed to a dean air (sham) atmosphere, and four to an atmosphere containing 1 ppm 03. PAM were obtained by lung lavage and placed on gold-colloid coated covers!ips. The area cleared of gold particles by migrating PAM after 24, 48, and 72 h was used as an indicator of cell mobility. The number of PAM recoverable by lavage was simitar for both groups (2 X 10s), but the percentage of macrophages that made tracks was significantly smaller with 95% certainty in the 03 group. For sham-exposed and 0-¡-exposed groups, the area cleared by PAM increased as the length of incubation increased, with the area cleared by the sham-exposed group being about 50% greater during each time period. When the two groups were compared statistically at each time point, the probability that they differed was, in each case, greater than 95%. it was concluded that the in vitro mlgratlonal potential of PAM was most likely decreased by in vivo exposure to O © 1981 by Hemisphere Publishing Corporation.

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