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A Gs-coupled purinergic receptor boosts Ca2+ influx and vascular contractility during diabetic hyperglycemia.

  • Author(s): Prada, Maria Paz
  • Syed, Arsalan U
  • Buonarati, Olivia R
  • Reddy, Gopireddy R
  • Nystoriak, Matthew A
  • Ghosh, Debapriya
  • Simó, Sergi
  • Sato, Daisuke
  • Sasse, Kent C
  • Ward, Sean M
  • Santana, Luis F
  • Xiang, Yang K
  • Hell, Johannes W
  • Nieves-Cintrón, Madeline
  • Navedo, Manuel F
  • et al.
Abstract

Elevated glucose increases vascular reactivity by promoting L-type CaV1.2 channel (LTCC) activity by protein kinase A (PKA). Yet, how glucose activates PKA is unknown. We hypothesized that a Gs-coupled P2Y receptor is an upstream activator of PKA mediating LTCC potentiation during diabetic hyperglycemia. Experiments in apyrase-treated cells suggested involvement of a P2Y receptor underlying the glucose effects on LTTCs. Using human tissue, expression for P2Y11, the only Gs-coupled P2Y receptor, was detected in nanometer proximity to CaV1.2 and PKA. FRET-based experiments revealed that the selective P2Y11 agonist NF546 and elevated glucose stimulate cAMP production resulting in enhanced PKA-dependent LTCC activity. These changes were blocked by the selective P2Y11 inhibitor NF340. Comparable results were observed in mouse tissue, suggesting that a P2Y11-like receptor is mediating the glucose response in these cells. These findings established a key role for P2Y11 in regulating PKA-dependent LTCC function and vascular reactivity during diabetic hyperglycemia.

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