Laser MicrobeamTargeting of Single NerveAxons in CellCulture
Published Web Location
https://doi.org/10.1007/978-1-4939-2152-2_16
Abstract
By focusing a laserlaser with short pulsespulses to a diffraction-limited spotdiffraction limited spot, single nervenerveaxonsaxons can be precisely targeted and injured. Subsequent repairrepair can be analyzed using various imagingimaging and biochemicalbiochemicaltechniquestechniques to understand the repair process. In this chapter, we will describe a roboticrobotic laser microscopemicroscopesystemlaser microscope system used to injure nerve axonsnerve axons while simultaneously observing repair using phasephase and fluorescencefluorescencemicroscopyfluorescence microscopy. We provide procedures for controlled laser targeting and an experimental approach for studying axonalaxonal repair in embryonicembryonicratrathippocampushippocampusneuronsneurons.
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