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Open Access Publications from the University of California

Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods.

  • Author(s): Gratton, Enrico
  • Breusegem, Sophie
  • Sutin, Jason
  • Ruan, Qiaoqiao
  • Barry, Nicholas
  • et al.

Published Web Location Commons 'BY' version 4.0 license

Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples.

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