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Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer.

  • Author(s): Yokoyama, WM
  • Koning, F
  • Stingl, G
  • Bluestone, JA
  • Coligan, JE
  • Shevach, EM
  • et al.
Abstract

We have produced a T cell hybridoma line by fusion of an IL-2-dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line. The resultant hybridoma was rapidly growing, lymphokine independent, and expressed T3 in association with the TCR gamma/delta heterodimer. Several subclones of the hybridoma line produced easily detectable levels of IL-2 after stimulation by anti-T3 or Con A. The availability of these cloned cell lines should greatly facilitate further functional, biochemical, and molecular studies of the TCR delta chain.

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