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Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy.

  • Author(s): Colomb, Florent;
  • Giron, Leila B;
  • Kuri-Cervantes, Leticia;
  • Adeniji, Opeyemi S;
  • Ma, Tongcui;
  • Dweep, Harsh;
  • Battivelli, Emilie;
  • Verdin, Eric;
  • Palmer, Clovis S;
  • Tateno, Hiroaki;
  • Kossenkov, Andrew V;
  • Roan, Nadia R;
  • Betts, Michael R;
  • Abdel-Mohsen, Mohamed
  • et al.
Abstract

A comprehensive understanding of the phenotype of persistent HIV-infected cells, transcriptionally active and/or transcriptionally inactive, is imperative for developing a cure. The relevance of cell-surface glycosylation to HIV persistence has never been explored. We characterize the relationship between cell-surface glycomic signatures and persistent HIV transcription in vivo. We find that the cell surface of CD4+ T cells actively transcribing HIV, despite suppressive therapy, harbors high levels of fucosylated carbohydrate ligands, including the cell extravasation mediator Sialyl-LewisX (SLeX), compared with HIV-infected transcriptionally inactive cells. These high levels of SLeX are induced by HIV transcription in vitro and are maintained after therapy in vivo. Cells with high-SLeX are enriched with markers associated with HIV susceptibility, signaling pathways that drive HIV transcription, and pathways involved in leukocyte extravasation. We describe a glycomic feature of HIV-infected transcriptionally active cells that not only differentiates them from their transcriptionally inactive counterparts but also may affect their trafficking abilities.

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