Skip to main content
eScholarship
Open Access Publications from the University of California

UC Santa Cruz

UC Santa Cruz Previously Published Works bannerUC Santa Cruz

Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase.

  • Author(s): Bolduc, JM;
  • Dyer, DH;
  • Scott, WG;
  • Singer, P;
  • Sweet, RM;
  • Koshland, DE;
  • Stoddard, BL
  • et al.
Abstract

Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View