Skip to main content
Download PDF
- Main
Mutagenesis and Laue Structures of Enzyme Intermediates: Isocitrate Dehydrogenase
Published Web Location
https://doi.org/10.1126/science.7761851Abstract
Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized.
Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.