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A protocol for full-rotation soft X-ray tomography of single cells
- Chen, Jian-Hua;
- Vanslembrouck, Bieke;
- Loconte, Valentina;
- Ekman, Axel;
- Cortese, Mirko;
- Bartenschlager, Ralf;
- McDermott, Gerry;
- Larabell, Carolyn A;
- Le Gros, Mark A;
- Weinhardt, Venera
Published Web Location
https://doi.org/10.1016/j.xpro.2022.101176Abstract
The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).
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