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Enzyme-catalyzed expressed protein ligation
- Henager, Samuel H;
- Chu, Nam;
- Chen, Zan;
- Bolduc, David;
- Dempsey, Daniel R;
- Hwang, Yousang;
- Wells, James;
- Cole, Philip A
Published Web Location
https://doi.org/10.1038/nmeth.4004Abstract
The enzyme subtiligase can be used to catalyze expressed protein ligation of proteins of interest with peptides lacking an N-terminal cysteine. This enables the analysis of protein modifications in the context of the native primary sequence.
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