- Main
Lipidomic profiling of influenza infection identifies mediators that induce and resolve inflammation.
- Author(s): Tam, Vincent C
- Quehenberger, Oswald
- Oshansky, Christine M
- Suen, Rosa
- Armando, Aaron M
- Treuting, Piper M
- Thomas, Paul G
- Dennis, Edward A
- Aderem, Alan
- et al.
Published Web Location
https://doi.org/10.1016/j.cell.2013.05.052Abstract
Bioactive lipid mediators play a crucial role in the induction and resolution of inflammation. To elucidate their involvement during influenza infection, liquid chromatography/mass spectrometry lipidomic profiling of 141 lipid species was performed on a mouse influenza model using two viruses of significantly different pathogenicity. Infection by the low-pathogenicity strain X31/H3N2 induced a proinflammatory response followed by a distinct anti-inflammatory response; infection by the high-pathogenicity strain PR8/H1N1 resulted in overlapping pro- and anti-inflammatory states. Integration of the large-scale lipid measurements with targeted gene expression data demonstrated that 5-lipoxygenase metabolites correlated with the pathogenic phase of the infection, whereas 12/15-lipoxygenase metabolites were associated with the resolution phase. Hydroxylated linoleic acid, specifically the ratio of 13- to 9-hydroxyoctadecadienoic acid, was identified as a potential biomarker for immune status during an active infection. Importantly, some of the findings from the animal model were recapitulated in studies of human nasopharyngeal lavages obtained during the 2009-2011 influenza seasons.
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