Structure of pig heart citrate synthase at 1.78 A resolution.
- Author(s): Larson, Steven B
- Day, John S
- Nguyen, Chieugiang
- Cudney, Robert
- McPherson, Alexander
- et al.
Published Web Locationhttps://doi.org/10.1107/s1744309109008343
Pig heart citrate synthase was crystallized from a small-molecule cocktail containing cystamine dihydrochloride, aspartame and benzamidine hydrochloride. The structure was refined to an R factor of 0.179 (R(free) = 0.222) using synchrotron data to a resolution of 1.78 A. The model includes the full-length protein, a chloride ion, a sulfate ion, 305 water molecules and an unexpected moiety attached through a disulfide linkage to Cys184, which was modeled as a half-cystamine molecule generated by disulfide exchange with the cystamine in the small-molecule cocktail.